nexbio_넥스바이오

제품

Plant pathogen detection kit

식물검역병원체진단

식물검역바이러스/바이로이드 qRT-PCR 진단키트 NEXBIO qRT-PCR kit(UDG system)는 핵산을 이용하여 대상 병원체의 존재여부를 검사할 수 있는 유전자 검사 제픔입니다. 본 제품은 1X one-step qRT-PCR mix, positive control, 8-strip tube로 구성되어 있으며, 1X one-step qRT-PCR mix는 유전자 증폭에 필요한 master mix, primer, probe가 혼합되어 reverse transcription 과 real-time PCR이 순차적으로 진행되도록 제작하였습니다. 따라서 1X one-step qRT-PCR mix 18 ul와 template 2 ul를 첨가하여 즉시 qRT-PCR 검사를 할 수 있으므로 사용이 간편합니다. qRT-PCR mix에 포함된 target probe는 Cy5, mutation probe는 HEX가 부착되어 있고, 제공해드린 positive control은 mutation이 되어 있으므로 증폭 곡선의 개수에 따라 양성대조구로 부터의 오염여부를 즉시 확인 할수 있습니다. 또한 UDG 시스템 적용으로 PCR product에 의한 오염발생의 가능성이 적습니다. real-time PCR 목록

No.	품명(Platform)	Code No.	규격	대상 병원체명	선발프라이머
1	NEXBIO AILV Real-time PCR kit (UDG system)	PB203	500T, 100T, 50T	Artichoke Italian latent virus (=Nepovirus italiaense)	AILV_F9B / AILV_R6B
2	NEXBIO AlMV Real-time PCR kit (UDG system)	PB202	500T, 100T, 50T	Alstroemeria mosaic virus (= Potyvirus alstromeriae)	AlMV_F3A / AlMV_R2A
3	NEXBIO APLPV Real-time PCR kit (UDG system)	PP201	500T, 100T, 50T	American plum line pattern virus (=Ilarvirus APLPV)	APLPV_F10B / APLPV_R9B
4	NEXBIO APLV Real-time PCR kit (UDG system)	PS201	500T, 100T, 50T	Andean potato latent virus	APLV_F1 / APLV_R2
5	NEXBIO APMoV Real-time PCR kit (UDG system)	PS202	500T, 100T, 50T	Andaen potato mottle virus (=Comovirus andesense)	APMoV_N40 / APMoV_C70
6	NEXBIO ArMV Real-time PCR kit (UDG system)	PS203	500T, 100T, 50T	Arabis mosaic virus (=Nepovirus arabis)	ArM_N24 / ArM_C32
7	NEXBIO BBrMV Real-time PCR kit (UDG system)	PP213	500T, 100T, 50T	Banana bract mosaic virus (=Potyvirus musae)	BBrMV_F7B / BBrMV_R11B
8	NEXBIO BBSSV Real-time PCR kit (UDG system)	PP207	500T, 100T, 50T	Blueberry shoestring virus (=Sobemovirus BSSV)	BBSSV_F3A / BBSSV_R1A
9	NEXBIO BBTV Real-time PCR kit (UDG system)	PP202	500T, 100T, 50T	Banana bunchy top virus (=Babuvirus musae)	BBTV_F2A / BBTV_R5A
10	NEXBIO BCMNV Real-time PCR kit (UDG system)	PS204	500T, 100T, 50T	Bean common mosaic necrosis virus (=Potyvirus phaseoli)	BCMNV_N30 / BCMNV_C30
11	NEXBIO BlScV Real-time PCR kit (UDG system)	PP206	500T, 100T, 50T	Blueberry scorch virus (=Carlavirus vaccinii)	BlScV_F5A / BlScV_R2A
12	NEXBIO BPMV Real-time PCR kit (UDG system)	PS205	500T, 100T, 50T	Bean pod mottle virus (=Comovirus siliquae)	BPMV_N10 / BPMV_C120
13	NEXBIO BRRV Real-time PCR kit (UDG system)	PP205	500T, 100T, 50T	BBlueberry red ringspot virus (=Soymovirus maculavaccinii)	BRRV_F2A / BRRV_R1A
14	NEXBIO CaCV Real-time PCR kit (UDG system)	PB205	500T, 100T, 50T	Capsicum chlorosis virus (=Capsicum chlorosis orthotospovirus) (=Orthotospovirus capsiciflavi)	CaCV_F9B / CaCV_R9B
15	NEXBIO CERV Real-time PCR kit (UDG system)	PB204	500T, 100T, 50T	Carnation etched ring virus (=Caulimovirus incidianthi)	CERV_F1A / CERV_R3A
16	NEXBIO CGMMV Real-time PCR kit (UDG system)	PS206	500T, 100T, 50T	Cucumber green mottle mosaic virus	CGMM_N30 / CGMM_C60
17	NEXBIO CLRV Real-time PCR kit (UDG system)	PS207	500T, 100T, 50T	Cherry leaf roll virus (=Nepovirus avii)	CLR_N60 / CLR_C10
18	NEXBIO CNFV Real-time PCR kit (UDG system)	PS208	500T, 100T, 50T	Carnation necrotic fleck virus (=Closterovirus necrodianthi)	CNF_N10 / CNF_C60
19	NEXBIO CPMMV Real-time PCR kit (UDG system)	PS209	500T, 100T, 50T	Cowpea mild mottle virus (=Carlavirus vignae)	CPMM_N10 / CPMM_C20
20	NEXBIO CRLV Real-time PCR kit (UDG system)	PS210	500T, 100T, 50T	Cherry rasp leaf virus (=Cheravirus avii)	CRLV_F2 / CRLV_R1
21	NEXBIO CRSV Real-time PCR kit (UDG system)	PS211	500T, 100T, 50T	Carnation ringspot virus (=Dianthovirus dianthi)	CRSV_N80 / CRSV_C20
22	NEXBIO CSNV Real-time PCR kit (UDG system)	PP208	500T, 100T, 50T	Chrysanthemum stem necrosis virus (=Orthotospovirus chrysanthinecrocaulis)	CSNV_F5A / CSNV_R3A
23	NEXBIO CYSDV Real-time PCR kit (UDG system)	PP209	500T, 100T, 50T	Cucurbit yellow stunting disorder virus (=Crinivirus cucurbitae)	CYSDV_F4A / CYSDV_R3A
24	NEXBIO FoMV Real-time PCR kit (UDG system)	PS212	500T, 100T, 50T	Foxtail mosaic virus (=Potexvirus setariae)	FoMV_Rep5 / FoMV_Rep3
25	NEXBIO GFLV Real-time PCR kit (UDG system)	PS213	500T, 100T, 50T	Grapevine fanleaf virus (=Nepovirus foliumflabelli)	GFLV_F100 / GFLV_R50
26	NEXBIO INSV Real-time PCR kit (UDG system)	PS214	500T, 100T, 50T	Impatiens necrotic spot virus (=Impatiens necrotic spot orthotospovirus) (=Orthotospovirus impatiensnecromaculae)	INSV_F4 / INSV_R3
27	NEXBIO IYSV Real-time PCR kit (UDG system)	PS215	500T, 100T, 50T	Iris yellow spot virus (=Iris yellow spot orthotospovirus) (=Orthotospovirus iridimaculaflavi)	IYS_N10 / IYS_C60
28	NEXBIO KGMMV Real-time PCR kit (UDG system)	PS216	500T, 100T, 50T	Kyuri green mottle mosaic virus	KGMM_N55 / KGMM_C63
29	NEXBIO LRSV Real-time PCR kit (UDG system)	PS217	500T, 100T, 50T	Lychnis ringspot virus (=Hordeivirus lychnis)	LRS_N30 / LRS_C60
30	NEXBIO PAMV Real-time PCR kit (UDG system)	PP210	500T, 100T, 50T	Potato aucuba mosaic virus (=Potexvirus marmoraucuba)	PAMV_F2A / PAMV_R3A
31	NEXBIO PDV Real-time PCR kit (UDG system)	PS218	500T, 100T, 50T	Prune dwarf virus (=Ilarvirus PDV)	PDV_F20 / PDV_C10
32	NEXBIO PEMV Real-time PCR kit (UDG system)	PS219	500T, 100T, 50T	Pea enation mosaic virus	PEM_N30 / PEM_C30
33	NEXBIO PepMV Real-time PCR kit (UDG system)	PS220	500T, 100T, 50T	Pepino mosaic virus (=Potexvirus pepini)	PepMV_N40 / PepMV_C70
34	NEXBIO PMTV Real-time PCR kit (UDG system)	PS221	500T, 100T, 50T	Potato mop-top virus (=Pomovirus solani)	PMT_N40 / PMT_C60
35	NEXBIO PRMV Real-time PCR kit (UDG system)	PS222	500T, 100T, 50T	Peach rosette mosaic virus (=Nepovirus persicae)	PRMV_N20 / PRMV_C80
36	NEXBIO PSTVd Real-time PCR kit (UDG system)	PS223	500T, 100T, 50T	Potato spindle tuber viroid (=Pospiviroid fusituberis)	PSTVd_F2 / PSTVd_R2
37	NEXBIO PYDV Real-time PCR kit (UDG system)	PP211	500T, 100T, 50T	Potato yellow dwarf virus (=Potato yellow dwarf alphanucleorhabdovirus) (=Alphanucleorhabdovirus tuberosum)	PYDV_F4A / PYDV_R4A
38	NEXBIO PZSV Real-time PCR kit (UDG system)	PS224	500T, 100T, 50T	Pelargonium zonate spot virus (=Anulavirus PZSV)	PZSV_F1 / PZSV_R4
39	NEXBIO RpRSV Real-time PCR kit (UDG system)	PS225	500T, 100T, 50T	Raspberry ringspot virus (=Nepovirus rubi)	RpRS_N35 / RpRS-C15
40	NEXBIO SBMV Real-time PCR kit (UDG system)	PS226	500T, 100T, 50T	Southern bean mosaic virus (=Sobemovirus SBMV)	SBM_N10 / SBM_C20
41	NEXBIO SLRSV Real-time PCR kit (UDG system)	PS227	500T, 100T, 50T	Strawberry latent ringspot virus (=Stralarivirus fragariae)	SLRSV_N20 / SLRSV_C50
42	NEXBIO SqMV Real-time PCR kit (UDG system)	PS228	500T, 100T, 50T	Squash mosaic virus (=Comovirus cucurbitae)	SqM_N30 / SqM-C45
43	NEXBIO TBRV Real-time PCR kit (UDG system)	PS229	500T, 100T, 50T	Tomato black ring virus (=Nepovirus nigranuli)	TBR_N20 / TBR_C70
44	NEXBIO TEV Real-time PCR kit (UDG system)	PP212	500T, 100T, 50T	Tobacco etch virus (=Potyvirus nicotianainsculpentis)	TEV_F7B / TEV_R7B
45	NEXBIO TICV Real-time PCR kit (UDG system)	PB201	500T, 100T, 50T	Tomato infectious chlorosis virus (=Crinivirus contagichlorosis)	TICV_F8B / TICV_R9B
46	NEXBIO TNV1 Real-time PCR kit (UDG system)	PP2031	500T, 100T, 50T	Tobacco necrosis virus	TNV_F4A / TNV_R5A
47	NEXBIO TNV2 Real-time PCR kit (UDG system)	PP2032	500T, 100T, 50T	Tobacco necrosis virus	TNV_F9B / TNV_R9B
48	NEXBIO ToBRFV Real-time PCR kit (UDG system)	PP204	500T, 100T, 50T	Tomato brown rugose fruit virus	ToBRFV_F7B / ToBRFV_R9B
49	NEXBIO ToBRFV(CSP1325) Real-time PCR kit (UDG system)	ISF201	500T, 100T, 50T		CSP1325_Fw / CSP1325_Rv
50	NEXBIO ToBRFV(CaTa28) Real-time PCR kit (UDG system)	ISF202	500T, 100T, 50T		CaTa28_Fw / CaTa28_Rv
51	NEXBIO ToBRFV(M&W) Real-time PCR kit (UDG system)	ISF203	500T, 100T, 50T		ToBRFVqs1 / ToBRFVqas2
52	NEXBIO ToRSV Real-time PCR kit (UDG system)	PS231	500T, 100T, 50T	Tomato ringspot virus (=Nepovirus lycopersici)	ToRSV_R1_N120 / ToRSV_R1_C20
53	NEXBIO TRSV Real-time PCR kit (UDG system)	PS232	500T, 100T, 50T	Tobacco ringspot virus (=Nepovirus nicotianae)	TRSV_N10 / TRSV_C80
54	NEXBIO TSV1 Real-time PCR kit (UDG system)	PS2331	500T, 100T, 50T	Tobacco streak virus (=Ilarvirus TSV)	TSN_N20 / TSN_C10
55	NEXBIO TSV2 Real-time PCR kit (UDG system)	PS2332	500T, 100T, 50T	Tobacco streak virus (=Ilarvirus TSV)	TS_N20 / TS_C30
56	NEXBIO TYMV Real-time PCR kit (UDG system)	PS234	500T, 100T, 50T	Turnip yellow mosaic virus (=Tymovirus brassicae)	TYM_N10 / TYM_C20
57	NEXBIO WSMV Real-time PCR kit (UDG system)	PS235	500T, 100T, 50T	Wheat streak mosaic virus (=Tritimovirus tritici)	WSMV_N30 / WSMV_C70
58	NEXBIO Cmi Real-time PCR kit (UDG system)	PP214	500T, 100T, 50T	Clavibacter michiganensis subsp. insidiosus	Cmi_F2A / Cmi_R6A
59	NEXBIO Cmn Real-time PCR kit (UDG system)	PP215	500T, 100T, 50T	Clavibacter michiganensis subsp. nebraskensis	Cmn_F8B / Cmn_R10B
60	NEXBIO BCTV Real-time PCR kit (UDG system)	PS301	500T, 100T, 50T	Beet curly top virus (=Curtovirus betae)	BCTV_F7B / BCTV_R6B
61	NEXBIO CLVd Real-time PCR kit (UDG system)	PS302	500T, 100T, 50T	Columnea latent viroid (=Pospiviroid latenscolumneae)	CLVd_F5A / CLVd_R4A
62	NEXBIO PCFVd Real-time PCR kit (UDG system)	PS303	500T, 100T, 50T	Pepper chat fruit viroid (=Pospiviroid parvicapsici)	PCFVd_F5A / PCFVd_R2A
63	NEXBIO TASVd Real-time PCR kit (UDG system)	PS305	500T, 100T, 50T	Tomato apical stunt viroid (=Pospiviroid apicimpeditum)	TASVd_F8A / TASVd_R1A
64	NEXBIO TCDVd Real-time PCR kit (UDG system)	PS306	500T, 100T, 50T	Tomato chlorotic dwarf viroid (=Pospiviroid chloronani)	TCDVd_F4A / TCDVd_R1A
65	NEXBIO ToLCNDV Real-time PCR kit (UDG system)	PS307	500T, 100T, 50T	Tomato leaf curl New Delhi virus (=Begomovirus solanumdelhiense)	ToLCNDV_F3A / TOLCNDV_R3A
66	NEXBIO TPMVd Real-time PCR kit (UDG system)	PS308	500T, 100T, 50T	Tomato planta macho viroid (=Pospiviroid machoplantae)	TPMVd_F1A / TPMVd_R4A
67	NEXBIO ToMMV(Cata9) Real-time PCR kit (UDG system)	ISF311	500T, 100T, 50T	Tomato mottle mosaic virus	CaTa9_Fw / CaTa9_Rv
68	NEXBIO ToMMV2 Real-time PCR kit (UDG system)	ISF312	500T, 100T, 50T		ToMMV2_Fw / ToMMV2_Rv
69	NEXBIO ToMMV(CataAT) Real-time PCR kit (UDG system)	ISF301	500T, 100T, 50T		CataAT_Fw / CataAT_Rv
70	NEXBIO Bc Real-time PCR kit (UDG system)	PP216	500T, 100T, 50T	Burkholderia caryophylli	BC-F5 / BC-R5
71	NEXBIO XAC Real-time PCR kit (UDG system)	PBA401	500T, 100T, 50T	xanthomonas arboricola pv.corylina	XAC 2-8F / XAC 2-8R
72	NEXBIO XAJ Real-time PCR kit (UDG system)	PBA402	500T, 100T, 50T	xanthomonas arboricola pv. Juglandis	XAJ 5F / XAJ 5R
73	NEXBIO XCM Real-time PCR kit (UDG system)	PBA403	500T, 100T, 50T	xanthomonas citri pv. Mangiferaeindicae	XCM5 2-1F / XCM5 2-1R
74	NEXBIO XVH Real-time PCR kit (UDG system)	PBA404	500T, 100T, 50T	Xanthomonas vasicola pv. Holcicola	XVH 1-2F / XVH 1-2R

RT-PCR 목록

No.	품명(Platform)	Code No.	규격	대상 병원체명	선발프라이머
1	NEXBIO GINV RT-PCR kit	NBQG2301	96T	Grapevine berry inner necrosis virus	GINV_F2A / GINV_R2A
2	NEXBIO GEVA RT-PCR kit	NBQG2302	96T	Grapevine emaravirus A	GEVA_F2A / GEVA_R2A
3	NEXBIO GLVd RT-PCR kit	NBQG2303	96T	Grapevine latent viroid	GLVd_F1A / GLVd_R3A
4	NEXBIO GLRaV13 RT-PCR kit	NBQG2304	96T	Grapevine leafroll-associated virus 13	GLRaV13_F1A / GLRaV13_R2A

구성품

품목	50 rxn	100 rxn	500 rxn
1X one-step qRT-PCR mix	25rxn × 2ea	25rxn × 4ea	25rxn × 20ea
Positive control	2ea	2ea	10ea
0.2ml tube(8-strip × 12)	1 pk(8-strip × 12)	1.5 pk(8-strip × 16)	6 pk(8-strip × 72)

Uracil-DNA Glycosylase (UDG) PCR diagnosis 실험과 같이 다량의 검체를 이용하여 PCR을 수행할 경우, carry-over contamination이 발생되어 정확한 진단을 어렵게 하는 경우가 생깁니다. Carry-over contamination란 제한된 공간에서 동일한 primer와 template를 이용하여 반복적인 PCR 반응을 수행하는 경우에 공기 등 다양한 경로(실험기구, 시약 등의 표면)를 통해 이전 PCR 산물이 새로운 PCR 반응 혼합액에 오염되는 현상을 말합니다.

NEXBIO UDG System에서는 PCR에 사용되는 dNTP mixture 중 dTTP 대신 dUTP를 사용하여 PCR을 수행합니다. UDG(Uracil-DNA Glycosylase)는 DNA 가닥 내 uracil과 deoxyribose 사이의 N-glycosidic 결합을 가수분해하는 효소로, uracil을 포함하는 단일 또는 이중가닥 DNA를 분해하여 PCR 반응이나 real-time PCR 수행 과정, 특히 분자진단과정에서 빈번하게 발생하는 carry-over contamina- tion에 의한 위양성 (false positive) 결과를 방지하고 정확하게 진단할 수 있도록 사용됩니다.