제품

식물검역병원체진단

식물검역바이러스/바이로이드 qRT-PCR 진단키트 NEXBIO qRT-PCR kit(UDG system)는 핵산을 이용하여 대상 병원체의 존재여부를 검사할 수 있는 유전자 검사 제픔입니다. 본 제품은 1X one-step qRT-PCR mix, positive control, 8-strip tube로 구성되어 있으며, 1X one-step qRT-PCR mix는 유전자 증폭에 필요한 master mix, primer, probe가 혼합되어 reverse transcription 과 real-time PCR이 순차적으로 진행되도록 제작하였습니다. 따라서 1X one-step qRT-PCR mix 18 ul와 template 2 ul를 첨가하여 즉시 qRT-PCR 검사를 할 수 있으므로 사용이 간편합니다. qRT-PCR mix에 포함된 target probe는 Cy5, mutation probe는 HEX가 부착되어 있고, 제공해드린 positive control은 mutation이 되어 있으므로 증폭 곡선의 개수에 따라 양성대조구로 부터의 오염여부를 즉시 확인 할수 있습니다. 또한 UDG 시스템 적용으로 PCR product에 의한 오염발생의 가능성이 적습니다. real-time PCR 목록

No.	품명(Platform)	Code No.	규격	대상 병원체명	선발프라이머
1	NEXBIO AILV qRT-PCR kit (UDG system)	PB203	500T, 100T, 50T	Artichoke Italian latent virus	AILV_F9B / AILV_R6B
2	NEXBIO AlMV qRT-PCR kit (UDG system)	PB202	500T, 100T, 50T	Alstroemeria mosaic viruss	AlMV_F3A / AlMV_R2A
3	NEXBIO APLPV qRT-PCR kit (UDG system)	PP201	500T, 100T, 50T	American plum line pattern virus	APLPV_F10B / APLPV_R9B
4	NEXBIO APLV qRT-PCR kit (UDG system)	PS201	500T, 100T, 50T	Andean potato latent virus	APLV_F1 / APLV_R2
5	NEXBIO APMoV qRT-PCR kit (UDG system)	PS202	500T, 100T, 50T	Andean potato mottle virus	APMoV_N40 /APMoV_C70
6	NEXBIO ArMV qRT-PCR kit (UDG system)	PS203	500T, 100T, 50T	Arabis mosaic virus	ArM_N24 / ArM_C32
7	NEXBIO BBrMV qRT-PCR kit (UDG system)	PP213	500T, 100T, 50T	Banana bract mosaic virus	BBrMV_F7B / BBrMV_R11B
8	NEXBIO BBSSV qRT-PCR kit (UDG system)	PP207	500T, 100T, 50T	Blueberry shoestring virus	BBSSV_F3A / BBSSV_R1A
9	NEXBIO BBTV qRT-PCR kit (UDG system)	PP202	500T, 100T, 50T	Banana bunchy top virus	BBTV_F2A / BBTV_R5A
10	NEXBIO BCMNV qRT-PCR kit (UDG system)	PS204	500T, 100T, 50T	Bean common mosaic necrosis virus	BCMNV_N30 / BCMNV_C30
11	NEXBIO BlScV qRT-PCR kit (UDG system)	PP206	500T, 100T, 50T	Blueberry scorch virus	BlScV_F5A / BlScV_R2A
12	NEXBIO BPMV qRT-PCR kit (UDG system)	PS205	500T, 100T, 50T	Bean pod mottle virus	BPMV_N10 / BPMV_C120
13	NEXBIO BRRV qRT-PCR kit (UDG system)	PP205	500T, 100T, 50T	Blueberry red ringspot virus	BRRV_F2A / BRRV_R1A
14	NEXBIO CaCV qRT-PCR kit (UDG system)	PB205	500T, 100T, 50T	Capsicum chlorosis virus	CaCV_F9B / CaCV_R9B
15	NEXBIO CERV qRT-PCR kit (UDG system)	PB204	500T, 100T, 50T	Carnation etched ring virus	CERV_F1A / CERV_R3A
16	NEXBIO CGMMV qRT-PCR kit (UDG system)	PS206	500T, 100T, 50T	Cucumber green mottle mosaic virus	CGMM_N30 / CGMM_C60
17	NEXBIO CLRV qRT-PCR kit (UDG system)	PS207	500T, 100T, 50T	Cherry leaf roll virus	CLR_N60 / CLR_C10
18	NEXBIO CNFV qRT-PCR kit (UDG system)	PS208	500T, 100T, 50T	Carnation necrotic fleck virus	CNF_N10 / CNF_C60
19	NEXBIO CPMMV qRT-PCR kit (UDG system)	PS209	500T, 100T, 50T	Cowpea mild mottle virus	CPMM_N10 / CPMM_C20
20	NEXBIO CRLV qRT-PCR kit (UDG system)	PS210	500T, 100T, 50T	Cherry rasp leaf virus	CRLV_F2 / CRLV_R1
21	NEXBIO CRSV qRT-PCR kit (UDG system)	PS211	500T, 100T, 50T	Carnation ringspot virus	CRSV_N80 / CRSV_C20
22	NEXBIO CSNV qRT-PCR kit (UDG system)	PP208	500T, 100T, 50T	Chrysanthemum stem necrosis virus	CSNV_F5A / CSNV_R3A
23	NEXBIO CYSDV qRT-PCR kit (UDG system)	PP209	500T, 100T, 50T	Cucurbit yellow stunting disorder virus	CYSDV_F4A / CYSDV_R3A
24	NEXBIO FoMV qRT-PCR kit (UDG system)	PS212	500T, 100T, 50T	Foxtail mosaic virus	FoMV_Rep5 / FoMV_Rep3
25	NEXBIO GFLV qRT-PCR kit (UDG system)	PS213	500T, 100T, 50T	Grapevine fanleaf virus	GFLV_F100 / GFLV_R50
26	NEXBIO INSV qRT-PCR kit (UDG system)	PS214	500T, 100T, 50T	Impatiens necrotic spot virus	INSV_F4 / INSV_R3
27	NEXBIO IYSV qRT-PCR kit (UDG system)	PS215	500T, 100T, 50T	Iris yellow spot virus	IYS_N10 / IYS_C60
28	NEXBIO KGMMV qRT-PCR kit (UDG system)	PS216	500T, 100T, 50T	Kyuri green mottle mosaic virus	KGMM_N55 / KGMM_C63
29	NEXBIO LRSV qRT-PCR kit (UDG system)	PS217	500T, 100T, 50T	Lychnis ringspot virus	LRS_N30 / LRS_C60
30	NEXBIO PAMV qRT-PCR kit (UDG system)	PP210	500T, 100T, 50T	Potato aucuba mosaic virus	PAMV_F2A / PAMV_R3A
31	NEXBIO PDV qRT-PCR kit (UDG system)	PS218	500T, 100T, 50T	Prune dwarf virus	PDV_F20 / PDV_C10
32	NEXBIO PEMV qRT-PCR kit (UDG system)	PS219	500T, 100T, 50T	Pea enation mosaic virus	PEM_N30 / PEM_C30
33	NEXBIO PepMV qRT-PCR kit (UDG system)	PS220	500T, 100T, 50T	Pepino mosaic virus	PepMV_N40 / PepMV_C70
34	NEXBIO PMTV qRT-PCR kit (UDG system)	PS221	500T, 100T, 50T	Potato mop-top virus	PMT_N40 / PMT_C60
35	NEXBIO PRMV qRT-PCR kit (UDG system)	PS222	500T, 100T, 50T	Peach rosette mosaic virus	PRMV_N20 / PRMV_C80
36	NEXBIO PSTVd qRT-PCR kit (UDG system)	PS223	500T, 100T, 50T	Potato spindle tuber viroid	PSTVd_F2 / PSTVd_R2
37	NEXBIO PYDV qRT-PCR kit (UDG system)	PP211	500T, 100T, 50T	Potato yellow dwarf virus	PYDV_F4A / PYDV_R4A
38	NEXBIO PZSV qRT-PCR kit (UDG system)	PS224	500T, 100T, 50T	Pelargonium zonate spot virus	PZSV_F1 / PZSV_R4
39	NEXBIO RpRSV qRT-PCR kit (UDG system)	PS225	500T, 100T, 50T	Raspberry ringspot virus	RpRS_N35 / RpRS-C15
40	NEXBIO SBMV qRT-PCR kit (UDG system)	PS226	500T, 100T, 50T	Southern bean mosaic virus	SBM_N10 / SBM_C20
41	NEXBIO SLRSV qRT-PCR kit (UDG system)	PS227	500T, 100T, 50T	Strawberry latent ringspot virus	SLRSV_N20 / SLRSV_C50
42	NEXBIO SqMV qRT-PCR kit (UDG system)	PS228	500T, 100T, 50T	Squash mosaic virus	SqM_N30 / SqM-C45
43	NEXBIO TBRV qRT-PCR kit (UDG system)	PS229	500T, 100T, 50T	Tomato black ring virus	TBR_N20 / TBR_C70
44	NEXBIO TEV qRT-PCR kit (UDG system)	PP212	500T, 100T, 50T	Tobacco etch virus	TEV_F7B / TEV_R7B
45	NEXBIO TICV qRT-PCR kit (UDG system)	PB201	500T, 100T, 50T	Tomato infectious chlorosis virus	TICV_F8B / TICV_R9B
46	NEXBIO TNV1 qRT-PCR (UDG system)	PP2031	500T, 100T, 50T	Tobacco necrosis virus	TNV_F4A / TNV_R5A
47	NEXBIO TNV2 qRT-PCR kit (UDG system)	PP2032	500T, 100T, 50T	Tobacco necrosis virus	TNV_F9B / TNV_R9B
48	NEXBIO ToBRFV qRT-PCR kit (UDG system)	PP204	500T, 100T, 50T	Tomato brown rugose fruit virus	ToBRFV_F7B / ToBRFV_R9B
49	NEXBIO ToBRFV(CSP1325) qRT-PCR kit (UDG system)	ISF201	500T, 100T, 50T		CSP1325_Fw / CSP1325_Rv
50	NEXBIO ToBRFV(CaTa28) qRT-PCR kit (UDG system)	ISF202	500T, 100T, 50T		CaTa28_Fw / CaTa28_Rv
51	NEXBIO ToBRFV(M&W) qRT-PCR kit (UDG system)	ISF203	500T, 100T, 50T		ToBRFVqs1 / ToBRFVqas2
52	NEXBIO ToRSV qRT-PCR kit (UDG system)	PS231	500T, 100T, 50T	Tomato ringspot virus	ToRSV_R1_N120 / ToRSV_R1_C20
53	NEXBIO TRSV qRT-PCR kit (UDG system)	PS232	500T, 100T, 50T	Tobacco ringspot virus	TRSV_N10 / TRSV_C80
54	NEXBIO TSV1 qRT-PCR kit (UDG system)	PS2331	500T, 100T, 50T	Tobacco streak virus	TSN_N20 / TSN_C10
55	NEXBIO TSV2 qRT-PCR kit (UDG system)	PS2332	500T, 100T, 50T	Tobacco streak virus	TS_N20 / TS_C30
56	NEXBIO TYMV qRT-PCR kit (UDG system)	PS234	500T, 100T, 50T	Turnip yellow mosaic virus	TYM_N10 / TYM_C20
57	NEXBIO WSMV qRT-PCR kit (UDG system)	PS235	500T, 100T, 50T	Wheat streak mosaic virus	WSMV_N30 / WSMV_C70
58	NEXBIO Cmi qRT-PCR kit (UDG system)	PP214	500T, 100T, 50T	Clavibacter michiganensis subsp. insidiosus	Cmi_F2A / Cmi_R6A
59	NEXBIO Cmn qRT-PCR kit (UDG system)	PP215	500T, 100T, 50T	Clavibacter michiganensis subsp. nebraskensis	Cmn_F8B / Cmn_R10B
60	NEXBIO BCTV qRT-PCR kit (UDG system)	PS301	500T, 100T, 50T	BCTV : Beet curly top virus	BCTV_F7B / BCTV_R6B
61	NEXBIO CLVd qRT-PCR kit (UDG system)	PS302	500T, 100T, 50T	CLVd : Columnea latent pospiviroid	CLVd_F5A / CLVd_R4A
62	NEXBIO PCFVd qRT-PCR kit (UDG system)	PS303	500T, 100T, 50T	PCFVd : Pepper chat fruit viroid	PCFVd_F5A / PCFVd_R2A
63	NEXBIO PepYLCIV qRT-PCR kit (UDG system)	PS304	500T, 100T, 50T	Pepper yellow leaf curl Indonesia virus	PPepYLCIV_F3A / PepYLCIV_R3A
64	NEXBIO TASVd qRT-PCR kit (UDG system)	PS305	500T, 100T, 50T	Tomato apical stunt viroid	TASVd_F8A / TASVd_R1A
65	NEXBIO TCDVd qRT-PCR kit (UDG system)	PS306	500T, 100T, 50T	Tomato chlorotic dwarf viroid	TCDVd_F4A / TCDVd_R1A
66	NEXBIO ToLCNDV qRT-PCR kit (UDG system)	PS307	500T, 100T, 50T	Tomato leaf curl New Delhi virus	ToLCNDV_F3A / TOLCNDV_R3A
67	NEXBIO TPMVd qRT-PCR kit (UDG system)	PS308	500T, 100T, 50T	Tomato planta macho viroid	TPMVd_F1A / TPMVd_R4A
68	NEXBIO ToMMV(CataAT) qRT-PCR kit (UDG system)	ISF301	500T, 100T, 50T	Tomato mottle mosaic virus	CataAT_Fw / CataAT_Rv

RT-PCR 목록

No.	품명(Platform)	Code No.	규격	대상 병원체명	선발프라이머
1	NEXBIO GINV RT-PCR kit	NBQG2301	96T	Grapevine berry inner necrosis virus	GINV_F2A / GINV_R2A
2	NEXBIO GEVA RT-PCR kit	NBQG2302	96T	Grapevine emaravirus A	GEVA_F2A / GEVA_R2A
3	NEXBIO GLVd RT-PCR kit	NBQG2303	96T	Grapevine latent viroid	GLVd_F1A / GLVd_R3A
4	NEXBIO GLRaV13 RT-PCR kit	NBQG2304	96T	Grapevine leafroll-associated virus 13	GLRaV13_F1A / GLRaV13_R2A

구성품

품목	50 rxn	100 rxn	500 rxn
1X one-step qRT-PCR mix	25rxn × 2ea	25rxn × 4ea	25rxn × 20ea
Positive control	2ea	2ea	10ea
0.2ml tube(8-strip × 12)	1 pk(8-strip × 12)	1.5 pk(8-strip × 16)	6 pk(8-strip × 72)

Uracil-DNA Glycosylase (UDG) PCR diagnosis 실험과 같이 다량의 검체를 이용하여 PCR을 수행할 경우, carry-over contamination이 발생되어 정확한 진단을 어렵게 하는 경우가 생깁니다. Carry-over contamination란 제한된 공간에서 동일한 primer와 template를 이용하여 반복적인 PCR 반응을 수행하는 경우에 공기 등 다양한 경로(실험기구, 시약 등의 표면)를 통해 이전 PCR 산물이 새로운 PCR 반응 혼합액에 오염되는 현상을 말합니다.

NEXBIO UDG System에서는 PCR에 사용되는 dNTP mixture 중 dTTP 대신 dUTP를 사용하여 PCR을 수행합니다. UDG(Uracil-DNA Glycosylase)는 DNA 가닥 내 uracil과 deoxyribose 사이의 N-glycosidic 결합을 가수분해하는 효소로, uracil을 포함하는 단일 또는 이중가닥 DNA를 분해하여 PCR 반응이나 real-time PCR 수행 과정, 특히 분자진단과정에서 빈번하게 발생하는 carry-over contamina- tion에 의한 위양성 (false positive) 결과를 방지하고 정확하게 진단할 수 있도록 사용됩니다.